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Cusabio β cgrp
β Cgrp, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B2+cgrp/Human+calcitonin+gene+related+peptide%2CCGRP+ELISA+Kit/pm40772530-166-51-56
Average 93 stars, based on 17 article reviews

β cgrp - by Bioz Stars, 2026-08

93/100 stars

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CGRP and cap promote cell proliferation but inhibit cell apoptosis in A549 cells grown under hyperoxic conditions. (A) Cell Counting Kit-8 assay revealed that hyperoxia inhibited A549 cell proliferation. (B) Different concentrations of CGRP (1 or 10 nM) and cap (1, 2.5, or 5 µM) promoted cell proliferation in the normal air group. (C) Different concentrations of CGRP (1, 10, or 100 nM) and cap (1, 2.5, or 5 µM) promoted cell proliferation under hyperoxia. (D) Flow cytometry revealed that hyperoxia promoted apoptosis of A549 cells, and both CGRP (10 nM) and cap (1 µM) reversed this. Data are presented as the mean ± SD of at least three repeats. *P<0.05, **P<0.01 and ***P<0.001. CGRP, calcitonin gene-related peptide; cap, capsaicin; PI, propidium iodine.

Journal: Molecular Medicine Reports

Article Title: Calcitonin gene‑related peptide alleviates hyperoxia‑induced human alveolar cell injury via the CGRPR/TRPV1/Ca2 + axis

doi: 10.3892/mmr.2024.13234

Figure Lengend Snippet: CGRP and cap promote cell proliferation but inhibit cell apoptosis in A549 cells grown under hyperoxic conditions. (A) Cell Counting Kit-8 assay revealed that hyperoxia inhibited A549 cell proliferation. (B) Different concentrations of CGRP (1 or 10 nM) and cap (1, 2.5, or 5 µM) promoted cell proliferation in the normal air group. (C) Different concentrations of CGRP (1, 10, or 100 nM) and cap (1, 2.5, or 5 µM) promoted cell proliferation under hyperoxia. (D) Flow cytometry revealed that hyperoxia promoted apoptosis of A549 cells, and both CGRP (10 nM) and cap (1 µM) reversed this. Data are presented as the mean ± SD of at least three repeats. *P<0.05, **P<0.01 and ***P<0.001. CGRP, calcitonin gene-related peptide; cap, capsaicin; PI, propidium iodine.

Article Snippet: When the cell confluence reached 50–60%, the cells were treated with 10 nM CGRP (cat. no. HY-P1548A; MedChemExpress) or 1 µM capsaicin (cat. no. HY-10448; MedChemExpress), and then stimulated with hyperoxia.

Techniques: Cell Counting, Flow Cytometry

Cgrp − / − mice exhibited reduced bone angiogenesis and lower bone formation, and bone mass than WT controls. (A) Agarose gel electrophoresis images of samples from wild type (WT) and Cgrp knockout (Cgrp−/−) mice. (B) Western blotting of CGRP, p‐FAK 397, p‐FAK 576, p‐FAK 925, FAK, p‐AKT, AKT, p‐ERK, ERK, VEGFA, and tubulin in HMEC‐1 cells with WT mice and CGRP−/− knockout mice. (C and D) Flow cytometry dot plot and quantification of CD31highEmcnhigh endothelial cells from WT and Cgrp−/− mice. (E and F) Representative images and quantification of CD31 (green)‐ and Emcn (red)‐stained femora from WT and Cgrp−/− mice. Scale bar, 100 µm. (G and H) Representative images and quantitation of Vefga (green) immunostaining in the femora of WT and Cgrp−/− mice. Scale bar, 100 µm. n = 3 mice in each group. (I–M) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT and Cgrp−/− mice. Scale bar, 1 mm. (N and O) Representative images of hematoxylin–eosin staining in distal femora and quantification of the number of adipocytes related to the tissue area. Scale bar, 100 µm. (P and Q) Representative images and quantitation of Runx2 (green) immunostaining in femora of WT and Cgrp−/− mice. Scale bar, 100 µm. (R and S) Representative images of TRAP staining and quantification of TRAP‐positive cells on the trabecular bone surfaces of WT and Cgrp−/− mice. Scale bar, 100 µm. n = 3 mice in each group. The data are shown as the mean ± standard deviation. * p < 0.05; ** p < 0.01; *** , and p < 0.001 by Student's t ‐test and one‐way analysis of variance (J–M). BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: Cgrp − / − mice exhibited reduced bone angiogenesis and lower bone formation, and bone mass than WT controls. (A) Agarose gel electrophoresis images of samples from wild type (WT) and Cgrp knockout (Cgrp−/−) mice. (B) Western blotting of CGRP, p‐FAK 397, p‐FAK 576, p‐FAK 925, FAK, p‐AKT, AKT, p‐ERK, ERK, VEGFA, and tubulin in HMEC‐1 cells with WT mice and CGRP−/− knockout mice. (C and D) Flow cytometry dot plot and quantification of CD31highEmcnhigh endothelial cells from WT and Cgrp−/− mice. (E and F) Representative images and quantification of CD31 (green)‐ and Emcn (red)‐stained femora from WT and Cgrp−/− mice. Scale bar, 100 µm. (G and H) Representative images and quantitation of Vefga (green) immunostaining in the femora of WT and Cgrp−/− mice. Scale bar, 100 µm. n = 3 mice in each group. (I–M) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT and Cgrp−/− mice. Scale bar, 1 mm. (N and O) Representative images of hematoxylin–eosin staining in distal femora and quantification of the number of adipocytes related to the tissue area. Scale bar, 100 µm. (P and Q) Representative images and quantitation of Runx2 (green) immunostaining in femora of WT and Cgrp−/− mice. Scale bar, 100 µm. (R and S) Representative images of TRAP staining and quantification of TRAP‐positive cells on the trabecular bone surfaces of WT and Cgrp−/− mice. Scale bar, 100 µm. n = 3 mice in each group. The data are shown as the mean ± standard deviation. * p < 0.05; ** p < 0.01; *** , and p < 0.001 by Student's t ‐test and one‐way analysis of variance (J–M). BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Article Snippet: The membranes were then blocked with 5% milk and incubated overnight with primary antibodies against p‐FAK (Tyr397) (Cell Signaling Technology, 3283), p‐FAK (Tyr576/577) (Cell Signaling Technology, 3281), p‐FAK (Tyr925) (Cell Signaling Technology, 3284), FAK (Cell Signaling Technology, 3285), p‐AKT (Ser473) (Cell Signaling Technology, 4060), AKT (Cell Signaling Technology, 9272), p‐ERK (Thr202/Tyr204) (Cell Signaling Technology, 9101), ERK (Cell Signaling Technology, 9102), VEGFA (Proteintech, 19003‐1‐AP), OPN (Proteintech, 22952‐1‐AP), BMP2 (Abcam, ab214821), Runx2 (Abcam, ab214821), β‐actin (Cell Signaling Technology, 4967), CGRP (Cell Signaling Technology, 14 959), Angptl4 (Proteintech, 18374‐1‐AP), Alpl (Proteintech, 11187‐1‐AP), Ocn (Proteintech, 16157‐1‐AP), Spp1 (Proteintech, 22952‐1‐AP), Sp7 (Proteintech, 28694‐1‐AP), Postn (Proteintech, 66491‐1‐Ig), Fabp4 (Proteintech, 12802‐1‐AP), Pparg (Proteintech, 16643‐1‐AP), Lpl (Proteintech, 28602‐1‐AP), Plin1 (Proteintech, 83905‐4‐RR), Adipoq (Proteintech, 83961‐4‐RR), Id4 (Proteintech, 21803‐1‐AP), GAPDH (Proteintech, 60004‐1‐Ig), and Tubulin (Cell Signaling Technology, 2146) at 4°C.

Techniques: Agarose Gel Electrophoresis, Knock-Out, Western Blot, Flow Cytometry, Staining, Quantitation Assay, Immunostaining, Micro-CT, Standard Deviation

During bone defect repair, Cgrp − / − mice had lower bone angiogenesis than WT mice and exhibited delayed bone regeneration. (A) Representative micro‐CT images of the trabecular microstructure of WT mice before and after bone defect modeling. (B and C) Representative images and quantification of PGP9.5 (green)‐ and CGRP (red)‐stained femora from WT and Cgrp−/− mice after bone defect introduction. Scale bar, 100 µm. (D and E) Representative images and quantification in PGP9.5 (green)‐ and CD31 (red)‐stained femora from WT and Cgrp−/− mice after bone defects. Scale bar, 100 µm. (F and G) Representative images and quantification of CD31 (green)‐ and Emcn (red)‐stained femora from WT and Cgrp−/− mice after bone defect introduction. Scale bar, 100 µm. n = 3 mice in each group. (H–L) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT and Cgrp−/− mice after bone defect introduction. Scale bar, 1 mm. (M) Representative images of hematoxylin–eosin staining in the bone generation area of WT and Cgrp−/− mice. Scale bar, 200 µm. (N and O) Representative images and quantitation of Runx2 (green) immunostaining in the bone generation area of WT and CGRP−/− mice. Scale bar, 100 µm. (P‐Q) Representative images of TRAP staining and quantification of TRAP‐positive cells on the trabecular bone surfaces of WT and CGRP−/− mice. Scale bar, 100 µm. n = 3 mice in each group. The data are shown as the mean ± standard deviation. * p < 0.05; ** p < 0.01; and *** p < 0.001 by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: During bone defect repair, Cgrp − / − mice had lower bone angiogenesis than WT mice and exhibited delayed bone regeneration. (A) Representative micro‐CT images of the trabecular microstructure of WT mice before and after bone defect modeling. (B and C) Representative images and quantification of PGP9.5 (green)‐ and CGRP (red)‐stained femora from WT and Cgrp−/− mice after bone defect introduction. Scale bar, 100 µm. (D and E) Representative images and quantification in PGP9.5 (green)‐ and CD31 (red)‐stained femora from WT and Cgrp−/− mice after bone defects. Scale bar, 100 µm. (F and G) Representative images and quantification of CD31 (green)‐ and Emcn (red)‐stained femora from WT and Cgrp−/− mice after bone defect introduction. Scale bar, 100 µm. n = 3 mice in each group. (H–L) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT and Cgrp−/− mice after bone defect introduction. Scale bar, 1 mm. (M) Representative images of hematoxylin–eosin staining in the bone generation area of WT and Cgrp−/− mice. Scale bar, 200 µm. (N and O) Representative images and quantitation of Runx2 (green) immunostaining in the bone generation area of WT and CGRP−/− mice. Scale bar, 100 µm. (P‐Q) Representative images of TRAP staining and quantification of TRAP‐positive cells on the trabecular bone surfaces of WT and CGRP−/− mice. Scale bar, 100 µm. n = 3 mice in each group. The data are shown as the mean ± standard deviation. * p < 0.05; ** p < 0.01; and *** p < 0.001 by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Techniques: Micro-CT, Staining, Quantitation Assay, Immunostaining, Standard Deviation

Recombinant Cgrp protein restored bone formation by promoting bone angiogenesis. (A and B) Representative images and quantification of CD31 (green)‐ and Emcn (red)‐stained femora from WT mice after bone defect introduction, and injection of the experimental group with recombinant Cgrp protein. Scale bar, 100 µm. (C and D) Representative images and quantitation of Vefga (green) immunostaining in the femora of WT and Cgrp−/− mice. Scale bar, 100 µm. (E‐I) Representative micro‐CT images and quantitative micro‐CT analysis of trabecular bone microarchitecture in WT mice after bone defect introduction, and injection of the experimental group with recombinant CGRP protein. Scale bar, 1 mm. (J) Representative images of hematoxylin–eosin staining in the bone generation areas of the PBS group and CGRP treatment group. (K and L) Representative images and quantitation of Runx2 (green) immunostaining in the bone generation areas of the phosphate‐buffered saline (PBS) group and CGRP treatment group. Scale bar, 100 µm. (M and N) Representative images of TRAP staining and quantification of TRAP‐positive cells on the trabecular bone surfaces of the PBS group and CGRP treatment group. Scale bar, 100 µm. n = 3 mice in each group. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and **** p < 0.0001by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: Recombinant Cgrp protein restored bone formation by promoting bone angiogenesis. (A and B) Representative images and quantification of CD31 (green)‐ and Emcn (red)‐stained femora from WT mice after bone defect introduction, and injection of the experimental group with recombinant Cgrp protein. Scale bar, 100 µm. (C and D) Representative images and quantitation of Vefga (green) immunostaining in the femora of WT and Cgrp−/− mice. Scale bar, 100 µm. (E‐I) Representative micro‐CT images and quantitative micro‐CT analysis of trabecular bone microarchitecture in WT mice after bone defect introduction, and injection of the experimental group with recombinant CGRP protein. Scale bar, 1 mm. (J) Representative images of hematoxylin–eosin staining in the bone generation areas of the PBS group and CGRP treatment group. (K and L) Representative images and quantitation of Runx2 (green) immunostaining in the bone generation areas of the phosphate‐buffered saline (PBS) group and CGRP treatment group. Scale bar, 100 µm. (M and N) Representative images of TRAP staining and quantification of TRAP‐positive cells on the trabecular bone surfaces of the PBS group and CGRP treatment group. Scale bar, 100 µm. n = 3 mice in each group. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and **** p < 0.0001by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Techniques: Recombinant, Staining, Injection, Quantitation Assay, Immunostaining, Micro-CT, Saline, Standard Deviation

CGRP regulates the secretion of ANGPTL4 by vascular endothelial cells. (A‐B) Representative images and quantification of Lepr (green)‐ and CD31 (red)‐stained femora from WT and CGRP−/− mice. Scale bar, 100 µm. (C) T‐SNE of ECs in mouse scRNA‐seq. (D) Comparison of ECs cluster 0/1/2 between high‐ and low‐CGRP group. (E) Ro/e analysis of ECs cluster 0/1/2 between high‐ and low‐CGRP group. (F) Markers genes in ECs cluster 0/1/2. (G) Expression of the Angptl4 gene in ECs cluster 0/1/2. (H) Volcano plot of differentially expressed genes between ECs cluster 0 and ECs cluster 1/2. (I) Enrichment analysis of differentially expressed genes between ECs cluster 0 and ECs cluster 1/2. (J–N) Correlation analysis between ANGPTL4 gene expression and CGRP gene expression in (J) GSE7158 , (K) GSE35959 , (L) GSE56814 , (M) GSE56815 , and (N) GSE62402 . (O and P) Number of differentially expressed genes upon treatment with CGRP. The red bar represents up‐regulated genes. The green bar represents down‐regulated genes. (Q) GO enrichment analysis of common up‐regulated and down‐regulated genes using the package clusterProfiler. The top 10 GO terms are displayed. (R) Fold change (log2) expression of up‐regulated genes associated with angiogenesis. The heat map is clustered in rows and columns. Scale bar, 100 µm. (S) mRNA expression of ANGPTL4 in HMEC‐1 cells with the control group, CGRP protein‐treated group, CGRP receptor (CLR/RAMP1) knockout group, CGRP receptor knockout + CGRP protein‐treated group. (T) Western blotting of Angptl4 and tubulin in HMEC‐1 cells with WT mice and CGRP−/− knockout mice. (U) ELISA analysis of ANGPTL4 secretion levels in the supernatant culture media of HMEC‐1 treated with CGRP or PBS for 24 h. (V and W) Representative images and quantification of Angptl4 (green)‐ and CD31 (red)‐stained femora from WT and CGRP−/− mice. Scale bar, 100 µm. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and **** p < 0.0001 by Student's t ‐test.

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: CGRP regulates the secretion of ANGPTL4 by vascular endothelial cells. (A‐B) Representative images and quantification of Lepr (green)‐ and CD31 (red)‐stained femora from WT and CGRP−/− mice. Scale bar, 100 µm. (C) T‐SNE of ECs in mouse scRNA‐seq. (D) Comparison of ECs cluster 0/1/2 between high‐ and low‐CGRP group. (E) Ro/e analysis of ECs cluster 0/1/2 between high‐ and low‐CGRP group. (F) Markers genes in ECs cluster 0/1/2. (G) Expression of the Angptl4 gene in ECs cluster 0/1/2. (H) Volcano plot of differentially expressed genes between ECs cluster 0 and ECs cluster 1/2. (I) Enrichment analysis of differentially expressed genes between ECs cluster 0 and ECs cluster 1/2. (J–N) Correlation analysis between ANGPTL4 gene expression and CGRP gene expression in (J) GSE7158 , (K) GSE35959 , (L) GSE56814 , (M) GSE56815 , and (N) GSE62402 . (O and P) Number of differentially expressed genes upon treatment with CGRP. The red bar represents up‐regulated genes. The green bar represents down‐regulated genes. (Q) GO enrichment analysis of common up‐regulated and down‐regulated genes using the package clusterProfiler. The top 10 GO terms are displayed. (R) Fold change (log2) expression of up‐regulated genes associated with angiogenesis. The heat map is clustered in rows and columns. Scale bar, 100 µm. (S) mRNA expression of ANGPTL4 in HMEC‐1 cells with the control group, CGRP protein‐treated group, CGRP receptor (CLR/RAMP1) knockout group, CGRP receptor knockout + CGRP protein‐treated group. (T) Western blotting of Angptl4 and tubulin in HMEC‐1 cells with WT mice and CGRP−/− knockout mice. (U) ELISA analysis of ANGPTL4 secretion levels in the supernatant culture media of HMEC‐1 treated with CGRP or PBS for 24 h. (V and W) Representative images and quantification of Angptl4 (green)‐ and CD31 (red)‐stained femora from WT and CGRP−/− mice. Scale bar, 100 µm. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and **** p < 0.0001 by Student's t ‐test.

Techniques: Staining, Comparison, Expressing, Gene Expression, Control, Knock-Out, Western Blot, Enzyme-linked Immunosorbent Assay, Standard Deviation

Endothelial cells and bone marrow mesenchymal stem cells maintain close interactions mediated by ANGPTL4. (A) Comparison of MSCs, OLCs, Chondrocytes, Fibroblasts, ECs, and Pericytes between high‐ and low‐CGRP group. (B) Ro/e analysis of various cell types between high‐ and low‐CGRP group. (C) Analysis of cell–cell communication between MSC and EC clusters. (D) Ligand‐receptor interactions in cell–cell communication between MSCs and EC clusters. (E–G) ANGPTL‐related signaling pathway network between MSCs and EC clusters. (H) Signaling role distribution heatmap of the ANGPTL‐related signaling pathway in cell‐cell communication analysis. (I and J) Bubble plot of (I) all pathways (J) ANGPTL signaling pathway interaction profiles across four cell populations (MSCs and EC clusters). Outgoing Interaction Strength: quantifying the cumulative signaling potential of each cell type as a sender. Incoming Interaction Strength: measuring the total signaling receptivity as a receiver. (K) Correlation analysis between RUNX2 transcriptional activity and ANGPTL4 expression levels, and comparison of RUNX2 transcriptional activity between high‐ and low‐BMD groups in GSE35959 . (L) Correlation analysis between SP7 transcriptional activity and ANGPTL4 expression levels, and comparison of SP7 transcriptional activity between high‐ and low‐BMD groups in GSE35959 . (M) Correlation analysis between RUNX2 transcriptional activity and ANGPTL4 expression levels, and comparison of RUNX2 transcriptional activity between high‐ and low‐BMD groups in GSE56814 . (N) Correlation analysis between SP7 transcriptional activity and ANGPTL4 expression levels, and comparison of SP7 transcriptional activity between high‐ and low‐BMD groups in GSE56814 . (O and P) RT‐qPCR analysis of Runx2, Postn, Osterix, Opn, Ocn, and Alpl expression levels in bone marrow mesenchymal stem cells (BMSCs) treated with different concentrations of recombinant Angptl4 protein. * p < 0.05, ** p < 0.01; and *** p < 0.001 by Student's t ‐test. (Q) Western blotting to determine Alpl, Runx2, Ocn, Spp1, Sp7, Postn, and GAPDH expression levels in BMSCs treated with 50 ng/mL recombinant Angptl4 protein. n = 3 mice in each group.

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: Endothelial cells and bone marrow mesenchymal stem cells maintain close interactions mediated by ANGPTL4. (A) Comparison of MSCs, OLCs, Chondrocytes, Fibroblasts, ECs, and Pericytes between high‐ and low‐CGRP group. (B) Ro/e analysis of various cell types between high‐ and low‐CGRP group. (C) Analysis of cell–cell communication between MSC and EC clusters. (D) Ligand‐receptor interactions in cell–cell communication between MSCs and EC clusters. (E–G) ANGPTL‐related signaling pathway network between MSCs and EC clusters. (H) Signaling role distribution heatmap of the ANGPTL‐related signaling pathway in cell‐cell communication analysis. (I and J) Bubble plot of (I) all pathways (J) ANGPTL signaling pathway interaction profiles across four cell populations (MSCs and EC clusters). Outgoing Interaction Strength: quantifying the cumulative signaling potential of each cell type as a sender. Incoming Interaction Strength: measuring the total signaling receptivity as a receiver. (K) Correlation analysis between RUNX2 transcriptional activity and ANGPTL4 expression levels, and comparison of RUNX2 transcriptional activity between high‐ and low‐BMD groups in GSE35959 . (L) Correlation analysis between SP7 transcriptional activity and ANGPTL4 expression levels, and comparison of SP7 transcriptional activity between high‐ and low‐BMD groups in GSE35959 . (M) Correlation analysis between RUNX2 transcriptional activity and ANGPTL4 expression levels, and comparison of RUNX2 transcriptional activity between high‐ and low‐BMD groups in GSE56814 . (N) Correlation analysis between SP7 transcriptional activity and ANGPTL4 expression levels, and comparison of SP7 transcriptional activity between high‐ and low‐BMD groups in GSE56814 . (O and P) RT‐qPCR analysis of Runx2, Postn, Osterix, Opn, Ocn, and Alpl expression levels in bone marrow mesenchymal stem cells (BMSCs) treated with different concentrations of recombinant Angptl4 protein. * p < 0.05, ** p < 0.01; and *** p < 0.001 by Student's t ‐test. (Q) Western blotting to determine Alpl, Runx2, Ocn, Spp1, Sp7, Postn, and GAPDH expression levels in BMSCs treated with 50 ng/mL recombinant Angptl4 protein. n = 3 mice in each group.

Techniques: Comparison, Activity Assay, Expressing, Quantitative RT-PCR, Recombinant, Western Blot

ANGPTL4 secreted by bone vessels restores bone formation by coupling with osteogenic differentiation of bone marrow mesenchymal stem cells. (A) RT‐qPCR analysis of Fabp4, Pparg, LPL, Id4, Adipoq, and perilipin expression levels in bone marrow mesenchymal stem cells (BMSCs) treated with different concentrations of recombinant Angptl4 protein. (B) Western blotting to determine Fabp4, Pparg, Lpl, Plin, Adipoq, Id4, and GAPDH expression levels in BMSCs treated with 50 ng/mL recombinant Angptl4 protein. n = 3 mice in each group. (C and D) Representative images of alizarin red S staining and quantification of calcification of BMSCs treated with recombinant Angptl4 protein. (E and F) Representative images of Alpl staining and quantification of Alpl activity in BMSCs treated with recombinant Angptl4 protein. (G and H) Representative images of oil red O staining and quantification of lipid formation by BMSCs treated with recombinant Angptl4 protein. (I–M) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT mice injected with recombinant Angptl4 protein after bone defect introduction. (N) Representative images of hematoxylin–eosin staining in the bone regeneration area of the PBS and Angptl4 treatment groups. (O) Schematic diagram of the effects of CGRP. Scale bar, 1 mm. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and *** p < 0.001 by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: ANGPTL4 secreted by bone vessels restores bone formation by coupling with osteogenic differentiation of bone marrow mesenchymal stem cells. (A) RT‐qPCR analysis of Fabp4, Pparg, LPL, Id4, Adipoq, and perilipin expression levels in bone marrow mesenchymal stem cells (BMSCs) treated with different concentrations of recombinant Angptl4 protein. (B) Western blotting to determine Fabp4, Pparg, Lpl, Plin, Adipoq, Id4, and GAPDH expression levels in BMSCs treated with 50 ng/mL recombinant Angptl4 protein. n = 3 mice in each group. (C and D) Representative images of alizarin red S staining and quantification of calcification of BMSCs treated with recombinant Angptl4 protein. (E and F) Representative images of Alpl staining and quantification of Alpl activity in BMSCs treated with recombinant Angptl4 protein. (G and H) Representative images of oil red O staining and quantification of lipid formation by BMSCs treated with recombinant Angptl4 protein. (I–M) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT mice injected with recombinant Angptl4 protein after bone defect introduction. (N) Representative images of hematoxylin–eosin staining in the bone regeneration area of the PBS and Angptl4 treatment groups. (O) Schematic diagram of the effects of CGRP. Scale bar, 1 mm. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and *** p < 0.001 by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Techniques: Quantitative RT-PCR, Expressing, Recombinant, Western Blot, Staining, Activity Assay, Micro-CT, Injection, Standard Deviation

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